View source for DNA Extraction and Gel Analysis Prelab

<h1>Pre-Lab Exercise: DNA Extraction and Gel Analysis Lab</h1>

<p><b>Print and hand in this exercise at the beginning of the DNA Extraction Lab. It will count as the quiz for the DNA Extraction Lab.</b></p>

<h2>DNA EXTRACTION FROM PLANTS AND ANIMALS</h2>
<ol>
<li>What type of cell is the human body made of?</li>
<li>Is it easier to extract DNA from a cheek cell or a fruit cell? Why?</li>
</ol>

<h2>DNA LOCATION & RELEASE</h2>
<ol start="3">
<li>Make a rough diagram showing all the barriers (membranes and walls) that need to be broken down in order to release DNA.</li>
<li>Label each membrane or wall.</li>
<li>Draw an X where the DNA should be found.</li>
<li>What do we use to degrade these barriers?</li>
<li>Explain how the substances above degrade the barriers.</li>
</ol>

<h2>EXTRACTION PROCEDURE</h2>
<ol start="8">
<li>What are the 3 steps for extracting DNA from a plant cell?</li>
<li>Which step would be skipped if we extracted DNA from a chicken liver? Why?</li>
</ol>

<h2>STRUCTURE OF CELL MEMBRANES</h2>
<ol start="10">
<li>Find in a reference book (biology) or online a representation of a phospholipid bilayer. Indicate and Label the lipid molecules and the proteins.</li>
</ol>

<h2>CRITICAL INGREDIENTS</h2>
<ol start="11">
<li>Why do we use soap?</li>
<li>Why do we use salt?</li>
<li>Why do we use alcohol?</li>
</ol>

<h2>DNA DIGESTION</h2>
<ol start="14">
<li>Why do we use restriction enzymes?</li>
<li>Name two factors that affect activity of enzymes.</li>
<li>What is a palindrome? Why is that term related to this experiment?</li>
<li>What is an incubator used for in general?</li>
<li>Why do we need one for this lab?</li>
</ol>

<h2>GEL ELECTROPHORESIS</h2>
<ol start="19">
<li>Why does the DNA molecule migrate through the gel?</li>
</ol>

<h2>BANDING PATTERN</h2>
<ol start="20">
<li>What is DNA sequencing?</li>
<li>How can it be used in forensics?</li>
</ol>

<ol start="22">
<h2><li>HOW LONG DID IT TAKE YOU TO COMPLETE THIS PRE-LAB?</li></h2>
</ol>